Services

Quantitative Genetics

CRISPR/Cas9

• Using CRISPR/Cas9 technology we provide services to generate gene-knockout of various cell lines of the desired gene of interest. We can also generate homo- or heterozygous gene knockouts.
• We generate cell lines with reduced expression of genes of interest using either stably-expressed shRNA or using CRISPR interference (CRISPRi) using lentiviral vectors in both cases.
• We provide genetically modified cell lines with increased expression for gene of interest of an exogenous gene/reporter. We can also generate cell lines with increased expression of the gene of interest from its endogenous genomic locus using CRISPR/Cas9-SAM (Synergistic Activation Mediator) with semi-tailored increase level of expression according to experimental needs.
• We can provide cell lines of interest with chromosomally-edited desired gene of interest, whether by tagging the gene or creating disease (or otherwise) gene mutations according to desired experimental paradigms.