Services

Proteomics

Mitochondrial and other proteins expression, purification, and proteomics core

• Biophysical characterization of purified proteins.
• Construction of fusion-tagged proteins either chromosomal or using exogenous plasmid vectors
• Affinity purification using protein specific antibody or epitope tags fused with bait proteins of interest to identify interacting partners through mass spectrometry.
• Broad range of chromatographic separation techniques, including:
  1. Affinity Chromatography
  2. Ion Exchange Chromatography
  3. Size Exclusion Chromatography
  4. Hydrophobic Interaction Chromatography
  5. Reversed Phase Chromatography
• Sample preparation (desalting, digestion, and fractionation).
• Custom packed LC column services for any chromatography separation type and dimension.
• Determination of intact proteins and protein identification from silver-stanning, SDS-PAGE or native gels.
• Protein identification in complex mixtures using a combination of easy Nano-LC and high-resolution mass spectrometer workflow.
• Protein and peptide identification using mass spectrometry-based proteomics and phosphoproteomics.
• Targeted identification and relative abundance of components.
• Identification and relative label-free quantitation of samples.
• Identification of cross-linked peptides/proteins.
• Identification of various post-translational modifications after phosphoprotein enrichment.
• Relative protein quantification based on label-fee, SILAC, or iTRAQ
• Identification and quantification of proteins, peptides, and post-translational modifications (through TiO2 and IMAC).