Training

A three-day workshop focused on modern bioanalytical techniques and will cover a variety of topics in MS-based proteomics such as quantitative proteomics, post-translational modifications (PTMs), and protein-protein interactions (PPIs). We are pleased to announce a new workshop on Modern Applications of Proteomics Techniques in Biology and Medicine This workshop will be held from the 20th until the 22nd of November, 2017.

This three-day workshop event is focused on practical applications of the use of modern bioanalytical techniques and will cover a variety of topics in MS-based proteomics such as quantitative proteomics, post-translational modifications (PTMs), and protein-protein interactions (PPIs). Hands-on training will include protein in-gel and in-solution digestion, peptide desalting, nLC-MS/MS sample processing, and protein identification. It will also offer an informal Question & Answer session.

The workshop represents a training opportunity for current and future users. It is open to students, active researchers and staff in the area of bio-analytical chemistry, mass spectrometry and proteomics. The workshop aims to promote state-of-the-art technologies and to facilitate collaborations within the scientific community across Canada.

The participants are offered an opportunity to process their own samples during the workshop. One sample will be LC-MS processed free of charge. Additional samples can be processed at current prices.

Topics covered:

INTRODUCTION TO PROTEOMICS

• Why perform proteomics?
• Proteomics Analysis (Workflow of a typical proteomics study)
• General principles of liquid chromatography-tandem mass spectrometry
• Tools (Nano LC ESI UHRMS)

CENTRAL ROLE OF MASS SPECTROMETRY

• Ultra High Resolution Mass Spectrometer (Fourier Transform-based Mass Spectrometry)
• FT Ion-Cyclotron Resonance
• FT Orbitrap
  • Orbitrap elite
  • Orbitrap Fusion (Lumos) Tribrid - An advanced MS in the field of proteomics
  • Multiple fragmentation methods

PROTEOMICS IN BIOLOGY AND MEDICINE

• Proteomics applications
• Multidimensional view of proteomes
• Quantitative Proteomics
• Quantitative proteomics techniques
• 2D-PAGE based method
• Label free MS method
• MS-based on stable isotope labeling method
• In vivo metabolic labeling (SILAC)
• In vitro labeling (iTRAQ, TMT, Proteolytic ¹⁸O labeling)
• Relative and absolute quantitation
• Experimental design and sample preparation
• Protein PTMs
• Introduction to PTMs
• Proteomic analysis of PTMs
• Protein phosphorylations
• Enrichment of phosphoproteins or phosphopeptides
• Analysis of phosphoproteins by mass spectrometry
• Protein-protein interactions
• Why protein-protein interactions?
• Experimental methods for discovering PPIs
• Fractionation-based methods
• Affinity-based methods
• Yeast two-hybrid
• AP-MS
• Databases and computational prediction of PPIs

A three-day workshop focused on practical applications of the use of advanced modern bioanalytical techniques covering a variety of topics in liquid chromatography, mass spectrometry and MS-based proteomics. We are pleased to announce a new workshop on Advances and Challenges in Liquid Chromatography-Mass Spectrometry-based Proteomics This workshop will be held from the 24th until the 26th of May, 2016.

This three-day workshop event is focused on practical applications of the use of modern bioanalytical techniques and will cover a variety of topics in liquid chromatography, mass spectrometry and MS-based proteomics. Hands-on training will include: protein in-gel and in-solution digestion, peptide desalting, nLC-MS/MS sample processing, and protein identification. It will also offer an informal Question & Answer session

The workshop represents a training opportunity for current and future users. It is open to students, active researchers and staff in the area of bio-analytical chemistry, mass spectrometry and proteomics. The workshop aims to promote state-of-the-art technologies and to facilitate collaborations within the scientific community across Canada.

The participants are offered an opportunity to process their own samples during the workshop. One sample will be LC-MS processed free of charge. Additional samples can be processed at current prices.

The application deadline is May 16th, 2016.

Topics covered:

LIQUID CHROMATOGRAPHY

• Chromatography: Basic Principles
• Theory of Liquid Chromatography (Retention, Selectivity, Efficiency, Resolution)
• Types of Liquid Chromatography
  • Ion Exchange Chromatography
  • Adsorption/Partition Chromatography (normal and reverse phase, HILIC)
  • Size Exclusion Chromatography
  • Affinity Chromatography
• Effects of pressure, column length, internal diameter and particle size in liquid chromatography
• From Liquid Chromatography to High Performance Liquid Chromatography
• Ultra High Performance Liquid Chromatography
• Nano Liquid (Ultra High Performance) Chromatography

MASS SPECTROMETRY

• Introduction
• Principles
• Instrumentation
• Sample Input System
• Ionization Sources (EI, CI, FAB, MALDI, ESI, APCI, APPI)
• Mass Analyzers (Time of flight, Magnetic Sector, FTICR, Quadrupole, Linear Ion Trap, 3D Ion Trap, Orbitrap)
• Detectors (FC, EM, SC, MCP)
• Specifications (Resolution, Accuracy, Sensitivity, Mass Range, Dynamic Range, Transmission)
• MS-MS (Tandem MS) Instruments
• Computer Based Data Acquisition and Processing System
• Mass Spectrum
• Structural Analysis (Isotope Patterns, Multiple Charging, Fragmentation Patterns)

PROTEOMICS

• Central Dogma of Biology
• Introduction to Proteomics
• Why Perform Proteomics?
• Proteomics Analysis
• 2D gel electrophoresis
• Protein Isolation and Fractionation
• Tools (Nano LC ESI UHRMS)
• EASY Nano-Ultra High Performance Liquid Chromatography
• Ultra High Resolution Mass Spectrometer (Fourier Transform-based Mass Spectrometry)
• FT-Orbitrap
• Orbitrap Fusion (Lumos) Tribrid - An advanced MS in the field of proteomics
• Multiple Fragmentation Methods
• Peptide Fragmentation
• Identification Methods/Bioinformatics
• Types of Proteomics

A four-day workshop focused on practical applications of the use of modern bioanalytical techniques covering a variety of topics in liquid chromatography, mass spectrometry and MS-based proteomics. The Liquid Chromatography Mass Spectrometry based Proteomics Workshop will be held from 13th until 16th of October, 2015.

A four-day workshop focused on practical applications of the use of modern bioanalytical techniques covering a variety of topics in liquid chromatography, mass spectrometry and MS-based proteomics. The Liquid Chromatography Mass Spectrometry based Proteomics Workshop will be held from 13th until 16th of October, 2015

Hands-on training will include:

• Protein in-gel and in-solution digestion
• Peptide desalting
• nLC-MS/MS sample processing
• Protein identification

It will also offer an informal Question & Answer session.

The workshop represents a training opportunity for current and future users. It is open to students, active researchers and staff in the area of bio-analytical chemistry, mass spectrometry and proteomics and aims to promote the state-of-the-art technologies and to facilitate collaborations within the scientific community across Canada.

Participants are offered an opportunity to process their own samples during the workshop. One sample will be LC-MS processed free of charge. Additional samples can be processed at current prices.

The application deadline is September 30th, 2015.

Topics covered:

LIQUID CHROMATOGRAPHY

• Chromatography: Basic Principles
• Theory of Liquid Chromatography (Retention, Selectivity, Efficiency, Resolution)
• Types of Liquid Chromatography
  • Ion Exchange Chromatography
  • Adsorption/Partition Chromatography (normal and reverse phase, HILIC)
  • Size Exclusion Chromatography
  • Affinity Chromatography
• Effects of pressure, column length, internal diameter and particle size in liquid chromatography
• From Liquid Chromatography to High Performance Liquid Chromatography
• Ultra High Performance Liquid Chromatography
• Nano Liquid (Ultra High Performance) Chromatography

MASS SPECTROMETRY

• Introduction
• Principles
• Instrumentation
• Sample Input System
• Ionization Sources (EI, CI, FAB, MALDI, ESI, APCI, APPI)
• Mass Analyzers (Magnetic Sector, Time of flight, Quadrupole, Ion Trap, FTICR)
• MS-MS (Tandem MS) Instruments
• Detectors
• Computer Based Data Acquisition and Processing System
• Mass Spectrum
• Mass Measurement
• Resolution
• Analysis (Isotope Patterns, Multiple Charging, Fragmentation Patterns)

PROTEOMICS

• Central Dogma of Biology
• Introduction to Proteomics
• Why Perform Proteomics?
• Proteomics Analysis
• 2D gel electrophoresis
• Mass Spectrometry for Proteomics
• Nano Liquid Chromatography-Ultra High Resolution Mass Spectrometer (Orbitrap Elite)
• Multiple Fragmentation Methods
• Peptide Fragmentation
• Identification Methods/Bioinformatics
• Types of Proteomics